Stereoelectronic effects in RNase-catalysed reactions of dinucleoside phosphate cleavage

Dimethyl Phosphate: Stereoelectronic versus Environmental Effects

Frank Alber,†,‡,§ Gerd Folkers,‡ and Paolo Carloni*,†,§,| International School for AdVanced Studies SISSA/ISAS, Via Beirut 4 I-34014 Trieste, Italy, Department of Pharmaceutical Chemistry, Swiss Federal Institute of Technology (ETH), CH-8057 Zürich, Switzerland, INFM-Istituto Nazionale per la Fisica della Materia, Italy, International Centre for Genetic Engineering and Biotechnology, AREA Scien...

Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants.

A transaminase from Halomonas elongata and four mutants generated by an in silico-based design were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives were excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with a high enantioselectivity. The different behaviour ...

The cleavage specificity of RNase

We determined sites in X ell mRNA that are cleaved by RNase III in the presence of X OOP antisense RNA, using a series of OOP RNAs with different internal deletions. In OOP RNA-cll mRNA structures containing a potential region of continuous double-stranded RNA bounded by a non-complementary unpaired region, RNase III cleaved the ell mRNA at one or more preferred sites located 10 to 14 bases fro...

Gold - Catalysed Reactions

In recent years, there have been three significant pieces of research which helped propel gold catalysis research into the forefront: the discoveries that gold/silica can catalyse the hydrogenation of pentene, that gold on carbon can be used in the hydrochlorination of acetylene and that deposition-precipitation (DP) methods can be used to prepare nanogold on titania capable of enabling the oxi...

Vibrational relaxation on hydrogen bonding in dinucleoside phosphate.